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Can leg pain and burning sensations affect an HIV test?

This Premium Q&A, reviewed and published, features a real conversation between an iCliniq user and a physician.

Patient's Query

Hi doctor,

I had tested with a fourth-generation duo rapid test kit at home after eight years of sexual exposure with a female sex worker, and it came back negative. The description of the test kit is as follows: common substances such as pain and fever medication, as well as blood components, may affect the performance of the 4th-generation antigen or antibody rapid test. So when I was tested with that kit at that time, I had no fever, but I had pain in my legs and a burning sensation in my hands and feet. So, do these symptoms affect the test result? Is this a false negative result? Kindly help me.

Thank you.

Answered by Dr. Rajesh Deshwal

Education:

MBBS

Professional Bio:

Dr. Rajesh Deshwal is a General Practitioner and HIV or AIDS specialist with 29 years of clinical experience. He completed his MBBS from Armed Forces Medical College, Pune, and then did his MD in Internal Medicine from Delhi University. He is currently working as Director and Chief Consultant at Shiv Ram Hospital in Pataudi, Gurugram, Haryana.

This doctor is not available for online consultations on the platform anymore.

Hello,

Welcome to icliniq.com.

The test result you received is completely accurate and reliable. Based on the information provided, if your potential exposure to HIV occurred eight years ago and your current test is negative, this strongly confirms that you are not infected with the human immunodeficiency virus (HIV). In such a case, there is no medical indication to repeat the test solely due to the past exposure, as HIV tests conducted after such a long period would definitely detect the virus if it were present.

Additionally, it is important to note that none of the symptoms you may be experiencing, the medications you are currently taking, or any abnormalities in your blood components have any effect on the accuracy or validity of the HIV test results. Modern HIV testing methods are highly specific and sensitive, and your result can be considered a true negative.

Thank you.

Patient's Query

Hi doctor,

Thank you for your answer.

While I was tested five months back, I took multivitamin tablets, B12 injection, Tryptomer, and Pregabalyn M 75 mg tablet for anxiety. I had tested my vitamin B12 level before one month of the HIV test; the B12 level was 2000 pg/mL. So does this affect my test result? Also, I was stressed and anxious for the last year before testing, and the result was not enough antibodies to detect HIV infection. Kindly answer.

Thank you.

Answered by Dr. Rajesh Deshwal

Education:

MBBS

Professional Bio:

Dr. Rajesh Deshwal is a General Practitioner and HIV or AIDS specialist with 29 years of clinical experience. He completed his MBBS from Armed Forces Medical College, Pune, and then did his MD in Internal Medicine from Delhi University. He is currently working as Director and Chief Consultant at Shiv Ram Hospital in Pataudi, Gurugram, Haryana.

This doctor is not available for online consultations on the platform anymore.

Hello,

Welcome back to icliniq.com.

Neither your drugs nor your stress and anxiety have to do anything with the test results.

Take care.

Patient's Query

Hi doctor,

Thank you for the reply.

I have attached the photo of the test kit, which is 100 % conclusive. However, while testing with the test kit, I accidentally opened the test device and came in contact with the kit device antigens (pinkish liquid) with a pricked finger; so, will these HIV antigens in the rapid test cause infection? Do I get HIV from coming into contact with antigens (gp41) of the test device? I am very scared. Also, I have continuous pain in my legs, a burning sensation in my legs, hands, and buttocks, and sometimes shifts to my knees.

Are these arthritis symptoms? Or have I developed peripheral neuropathy? Or other diseases which may have symptoms like continuous pain in my legs, burning sensation in hands, buttocks, knees, and on my back, which the HIV antibody test kits can not detect? For the past year, I have been under stress and fear of HIV. So kindly explain in brief. Is this test 100 % reliable, or do I need more tests? Do the rapid tests detect HIV infection after eight years of exposure?

Thank you.

Answered by Dr. Rajesh Deshwal

Education:

MBBS

Professional Bio:

Dr. Rajesh Deshwal is a General Practitioner and HIV or AIDS specialist with 29 years of clinical experience. He completed his MBBS from Armed Forces Medical College, Pune, and then did his MD in Internal Medicine from Delhi University. He is currently working as Director and Chief Consultant at Shiv Ram Hospital in Pataudi, Gurugram, Haryana.

This doctor is not available for online consultations on the platform anymore.

Hello,

Welcome back to icliniq.com.

I saw the photos you have attached (attachments removed to protect the patient's identity). You do not get human immunodeficiency virus (HIV) by touching test kit reagents or antigens. The symptoms that you are mentioning are due to anxiety, stress, and depression. You are 100% negative for HIV. I suggest you seek the opinion of a psychologist or psychiatrist for your psychological issues.

I hope this helps.

Kindly revert so I can assist you further.

Thank you.

Patient's Query

Hi doctor,

Thank you for your answer.

Do HIV 4th-generation rapid tests detect HIV infection after eight years of exposure? I tested CBC four months before the HIV test, and the report showed that lymphocytes were 15 %, and after the test, the lymphocytes were 20 %. Do low lymphocytes affect the test result?

On my left-hand thumb, I have two vertical grey lines, which I read somewhere is a symptom of HIV. And also in the last eight years, I have had armpit lumps three to four times; all these symptoms scared me, and I thought the result was a false negative. Do I need more testing?

Thank you.

Answered by Dr. Rajesh Deshwal

Education:

MBBS

Professional Bio:

Dr. Rajesh Deshwal is a General Practitioner and HIV or AIDS specialist with 29 years of clinical experience. He completed his MBBS from Armed Forces Medical College, Pune, and then did his MD in Internal Medicine from Delhi University. He is currently working as Director and Chief Consultant at Shiv Ram Hospital in Pataudi, Gurugram, Haryana.

This doctor is not available for online consultations on the platform anymore.

Hello,

Welcome back to icliniq.com.

You have done enough testing. I suggest you not perform any laboratory tests. I also suggest you live your life and not waste it by thinking about inconsequential things. You are negative for HIV.

I hope this helps.

Kindly revert so I can assist you further.

Thank you.

Medically reviewed by iCliniq medical review team
Published At August 9, 2022
Reviewed At November 25, 2025

Education:

MBBS

Professional Bio:

Dr. Rajesh Deshwal is a General Practitioner and HIV or AIDS specialist with 29 years of clinical experience. He completed his MBBS from Armed Forces Medical College, Pune, and then did his MD in Internal Medicine from Delhi University. He is currently working as Director and Chief Consultant at Shiv Ram Hospital in Pataudi, Gurugram, Haryana.

This doctor is not available for online consultations on the platform anymore.

Same symptoms don't mean you have the same problem. Consult a doctor now!

Education:

MBBS

Professional Bio:

Dr. Rajesh Deshwal is a General Practitioner and HIV or AIDS specialist with 29 years of clinical experience. He completed his MBBS from Armed Forces Medical College, Pune, and then did his MD in Internal Medicine from Delhi University. He is currently working as Director and Chief Consultant at Shiv Ram Hospital in Pataudi, Gurugram, Haryana.

This doctor is not available for online consultations on the platform anymore.

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